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. 2019 May 6;9:6910. doi: 10.1038/s41598-019-43402-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The Second Trial relative expression values of blood-cell neuroplasticity-related genes. The relative expression levels of NGFRP (P ≤ 0.01), VEGF-A (P ≤ 0.001), BDNF (P ≤ 0.01), IGF1 (P ≤ 0.01) and NGF (P ≤ 0.05) genes of EE group (white) showed a significant difference between T1 versus T2 (as in the First Trial). While in EEplus group (grey) relative expression values revealed no significant differences between T1, T2, and T3. All genes included in the study presented a recovery time sufficient to return to baseline.