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. 2019 May 6;9:6917. doi: 10.1038/s41598-019-43294-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A JAK inhibitor, tofacitinib, suppressed the invasiveness of PC-3 cells induced by IL-7. (A) PC-3 cells were treated with a JAK inhibitor, tofacitinib (100 ng/mL), for 1 h, followed by IL-7 (100 ng/mL) treatment for 30 min, and were then subjected to immunoblot analysis of STAT5 phosphorylation. (B) PC-3 cells were left to invade through matrigel (250 μg/mL) for 24 h after treatment with IL-7 (10 ng/mL) with or without tofacitinib (100 ng/mL). Invading cells were stained with crystal violet (upper panel), counted using ImageJ software, and plotted as a graph (lower panel). (C) Dil-labeled cells were left to invade through vertical collagen gel (2 mg/mL) for 18 h after treatment with IL-7 (10 ng/mL) with or without of tofacitinib (100 ng/mL). Representative image of cells invading vertical collagen gel (upper panel) and dot graph showing the maximum distances of the invading cells (lower panel). Scale bar = 0.5 mm. Bars indicate means. P-values were done by the Mann–Whitney U test (B,C). Results represent two or three independent experiments.