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. 2019 May 6;9:6927. doi: 10.1038/s41598-019-43472-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of apoptosis in HSF1 KO XX medaka. (A–H) Apoptosis with TUNEL staining (green) and nuclear DAPI staining (blue) in gonadal regions of WT XX (A,E), HT-treated WT XX (B,F), HSF1 KO XX (C,G), and HT-treated HSF1 KO XX (D,H) fry. White dot, gonadal regions; white arrow, apoptosis in gonadal regions. Scale bar, 30 μm. n = 4. (I) Rate of apoptotic germ cells (TUNEL-positive germ cells over total germ cells). (J) Rate of apoptotic gonadal somatic cells (TUNEL-positive gonadal somatic cells over total gonadal somatic cells). (K,L) Number of germ cells (K) or gonadal somatic cells (L) of WT (+/+) and KO (−/−) fry at 0 dph. **p < 0.01.