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. 2019 May 6;9:6909. doi: 10.1038/s41598-019-43335-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased myocardial triglyceride accumulation in Plin2^−/− hearts. (A) Triglyceride and (B) diglyceride content in hearts of 8-week-old Plin2^+/+ and Plin2^−/− mice after 4 hours fasting (n = 6–7). (C) Triglyceride content in cardiomyocytes isolated from Plin2^+/+ and Plin2^−/− hearts after O/N fast (n = 5–7). (D) Triglyceride content in fibroblasts isolated from Plin2^+/+ and Plin2^−/− hearts after O/N fast (n = 4). (E) Triglyceride content in liver of Plin2^+/+ and Plin2^−/− mice after 4 hours fasting (n = 4–5). (F) Confocal microscopy images on Plin2^+/+ and Plin2^−/− cardiomyocytes, shown as a merged picture containing 10 z-stacks compressed into one image. LDs are shown in red and nuclei in blue (x630 magnification, scale bar: 10 µm). (G) Quantification of ORO positive area (pixels) in Plin2^+/+ and Plin2^−/− cardiomyocytes (n = 4–5). Data are presented as mean ± SEM, *p < 0.05 vs. Plin2^+/+, **p < 0.01 vs. Plin2^+/+ and ***p < 0.001 vs. Plin2^+/+; TG, triglycerides; DG, diglyceride; O/N, overnight.