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. 2019 May 6;9:6923. doi: 10.1038/s41598-019-43356-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fecal microbial composition at the Phylum Level. Stool samples were collected as described in Fig. 1 legend and microbial composition determined by 16 S rRNA V4 hypervariable sequencing using Illumina MiSeq System. (A) Heat maps represent percent total Operational Taxonomic Units (OTUs) identified in each sample (Naïve C57BL/6; C57BL/6.1-C57BL/6.4) (n = 4); (C57BL/6 EAE; CPEAE.1-CPEAE.5) (n = 5); (Naïve SJL/J; SJL/J.1-SJL/J.3) (n = 3) and (SJL/J EAE; RREAE.1-RREAE.3) (n = 3) and were generated using Genesis software. (B) Mean percent OTU abundance represented as pie chart for each group. (C–F) One-way ANOVA, followed by Tukey’s multiple comparisons, was performed in order to assess significance in the indicated phyla. Bars represent mean ± SEM and exact p-values indicated in text (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).