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. 2019 May 6;9:6991. doi: 10.1038/s41598-019-43518-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LIG increases bone mass in vivo. (a) Calcein staining of zebrafish larvae at 9 dpf. Scale bars, 500 μm. Arrowheads, bone calcein staining. (b) Number of calcein-stained vertebrae in zebrafish at 9 dpf. Error bars indicate s.e.m., n = 13 for each group. ^###P ≤ 0.001 vs. control group. **P ≤ 0.01, ***P ≤ 0.001 vs. model group. (c) The expression mRNA for Alp, Runx2a, sp7, and BMP2b were measured by quantitative RT-PCR in zebrafish at 9 dpf. Error bars indicate s.e.m. (n = 4). ^#P ≤ 0.05, ^###P ≤ 0.001 vs. control group. *P ≤ 0.05, **P ≤ 0.01 vs. model group.