Figure 4.

LIG attenuates H₂O₂-induced apoptosis and promotes differentiation in OBs through GPR30. (a) Cell viability was measured by MTT reduction in OBs with LIG, G15 and ICI182780 in the presence of H₂O₂. Error bars indicate s.e.m. (n = 6); ^##P ≤ 0.01, ^###P ≤ 0.001 vs. control group. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. model group. (b) Representative immunoblotting images for Bcl-2 in MC3T3-E1 cells with LIG and G15 under oxidative stress. (c) Alizarin red staining of BMSCs after 14 days (D14) in culture under OIM with LIG and co-treated with 100 nM G15, 100 nM ICI182780 or 100 nM SNG1153 over the entire culture period.