Table 2.

2³ full factorial designs used to optimize rhamnolipid production

Factorial design 1
Treatment	Canola oil (% v/v)	(NH4)2SO4 (g/L)	Production time (days)
T1	1	1	7
T2	3	1	7
T3	1	4	7
T4	3	4	7
T5	1	1	14
T6	3	1	14
T7	1	4	14
T8	3	4	14

Factorial design 2
Treatment	Waste canola oil (% v/v)	(NH4)2SO4 (g/L)	Production time (days)
T1	1	1	7
T2	3	1	7
T3	1	4	7
T4	3	4	7
T5	1	1	14
T6	3	1	14
T7	1	4	14
T8	3	4	14

Factorial design 3
Treatment	Canola oil (% v/v)	NaNO3 (g/L)	Production time (days)
T1	1	1	7
T2	3	1	7
T3	1	4	7
T4	3	4	7
T5	1	1	14
T6	3	1	14
T7	1	4	14
T8	3	4	14

Factorial design 4
Treatment	Waste canola oil (% v/v)	NaNO3 (g/L)	Production time (days)
T1	1	1	7
T2	3	1	7
T3	1	4	7
T4	3	4	7
T5	1	1	14
T6	3	1	14
T7	1	4	14
T8	3	4	14