Figure 1.

Analysis of the global transcriptional changes, determined by RNA-seq, during heat shock in L. major promastigotes. (A) PCA plot of the three replicates (r1–r3) derived from each experimental condition (26 or 37 °C). (B) Differential expression analysis of the 10,700 transcripts annotated in the L. major genome. The plot shows the relationship between the mean of the normalized counts and the fold change (log₂) between 26 and 37 °C conditions. Each point represents one transcript. Points colored in red or blue represent transcripts significantly up- or down-expressed at 37 °C, respectively; points colored in black correspond to transcript showing a constant expression in both conditions.