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. 2019 May 6;9:6919. doi: 10.1038/s41598-019-43354-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temperature-dependent expression levels for the transcripts derived from the HSP70 locus. Alignment on the L. major HSP70 gene locus of the RNA-seq reads obtained from RNA samples (three biological replicates) isolated from either promastigotes growth at 26 °C (blue) or heat shocked at 37 °C (red). The position of the two transcripts, LmjF.28.T2270 and LmjF.28.T2280 (black arrows), transcribed from the HSP70 locus, is indicated together with the location of their corresponding CDS (grey arrows). Coverage (Cov.) is expressed as counts per million of reads (CPM).