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. 2019 May 6;9:6919. doi: 10.1038/s41598-019-43354-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temperature-dependent expression of transcript LmjF.28.T3032, which encodes for a previously non-annotated protein. (A) Alignment of the RNA-seq reads derived from RNA samples (three biological replicates) isolated from either promastigotes growth at 26 °C (blue) or heat shocked at 37 °C (red). The location of the transcript is indicated by a black arrow, whereas the location of the putative ORF is shown by a grey arrow. Coverage (Cov.) is expressed as counts per million of reads (CPM). (B) Analysis by Northern blotting of transcript LmjF.28.T3032 in RNA samples derived from parasites incubated at 26 or 37 °C. The sizes (in nucleotides) of the L. major rRNA molecules⁷² used as molecular markers are indicated. Bottom, ethidium bromide (EtBr) staining of the gel, before membrane transfer and hybridization. Uncropped images shown in Supplementary Figure 1. (C) Sequence alignment between the protein ABB37_01471 of L. pyrrhocoris and the putative protein encoded in transcript LmjF.28.T3032 (LmjF.28.P3032).