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. 2019 Apr 30;10:272. doi: 10.3389/fendo.2019.00272

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Copyright © 2019 Banerjee, Chakraborty, Chakraborty, Ghosh and Jana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Graphical representation of the level of (A) intra-testicular Testosterone (ng/ml) and (B) serum Testosterone (ng/ml). (C) Western blot and (D) RT-PCR of CYP11A1, StAR, 3β HSD, 17β HSD, SF1, and DAX1 with isolated testicular Leydig cells. (E) Western blot of AhR and CYP1A1 with isolated Leydig cells. β Actin was used as the loading control. (F) ROS generation in isolated testicular Leydig cells; Western blot of phospho and complete p38 MAPK (lower panel). At least three independent experiments were performed. Histogram is expressing the value of, mean ± SEM. One-way ANOVA was followed by Dunnett multiple comparison test. The level of significance was set at ^***(P < 0.001) as compared with control. B(a)P, Benzo(a)pyrene (5 mg/kg); Res, Resveratrol (50 mg/kg).