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. 2019 Apr 30;10:520. doi: 10.3389/fpls.2019.00520

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Copyright © 2019 Yu, Gao, Liu, Song, Xiao, Liu, Hou and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional Classification of the DEGs by Gene Ontology (GO) Enrichment Analysis. RNA was extracted from the BrAN3-silencing leaves with the empty vector pTY-S-inoculated leaves as the control at 60 days post inoculation (DPI), and used for library construction and RNA-Seq analysis. GO enrichment analysis of the DEGs was implemented by the GOseq R packages based on the Wallenius non-central hyper-geometric distribution (Young et al., 2010), which allows the adjustment for gene length bias in DEGs. It assigned all the genes and the DEGs to the three GO categories: biological processes, cell components, and molecular functions.