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. 2019 Apr 30;10:520. doi: 10.3389/fpls.2019.00520

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Copyright © 2019 Yu, Gao, Liu, Song, Xiao, Liu, Hou and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the expression of 9 out of the 41 differential expression genes (DEGs) by real-time RT-PCR. Gene-specific primers were designed to target all the homologous sequences of each gene in Chinese cabbage. Relative expression levels were calculated using the standard curve method with BrAct (Bra028615; Dheda et al., 2004) as the internal control gene. The FPKM expression levels were calculated as fragments per kilobase of exon model per million mapped (FPKM) values. Bars represent the means of three replicates ± standard errors (vertical bars). Genes: 1, Bra011332; 2, Bra014411; 3, Bra023958; 4, Bra027232; 5, Bra003044; 6, Bra022663; 7, Bra005136; 8, Bra017136; 9, Bra025708. Asterisks indicate a significant difference between pTY-S- and pTY-BrAN3-inoculated plants at p ≤ 0.01 as calculated by t-test.