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. 2019 Apr 30;10:399. doi: 10.3389/fneur.2019.00399

Figure 10.

Figure 10

Effect of cardamom oil on amyloid β expression in cortex tissue (400X). (a) Normal control: Cortex: showing normal neuronal cells without immunoreactivity (arrow){Immunostaining: Amyloid- β, 400X}, (b) AlCl3 group: Cortex: showing significantly enhanced expression of amyloid- β, exhibited by brown coloration at cortical neurons {Immunostaining: Amyloid- β, 400X}, (c) AlCl3 + Donepezil hydrochloride (1 mg/kg): Cortex: showing normal histology, normal neuronal cells without immunoreactivity (arrow) {Immunostaining: Amyloid- β, 400X}, (d) AlCl3 + Cardamom oil (100 mg/kg): Cortex: showing normal histology, significantly enhanced expression of amyloid- β, exhibited by brown coloration at cortical neurons (arrow) {Immunostaining: Amyloid- β, 400X}, (e) AlCl3 + Cardamom oil (200 mg/kg): Cortex: showing normal neuronal cells without immunoreactivity at cortex (small arrow), note weak immunoreactivity (large arrow){Immunostaining: Amyloid- β, 400X}, (f) Histogram of IHC. Data are expressed as mean ± SEM (n = 6), #p < 0.05 when compared with normal control, *p < 0.05 when compared with disease control group. The magnification was 400 X. AlCl3, Aluminum chloride; SEM, Standard Error of Mean; IHC, Immunohistochemistry.