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. 2019 Apr 30;10:915. doi: 10.3389/fimmu.2019.00915

Figure 4.

Figure 4

Pericellular proteolysis of bovine MØs upon challenge with N. caninum and T. gondii. Gelatin degradation of MØs challenged with N. caninum Nc-Spain7 (Nc-Spain7-MØ), Nc-Spain1H (Nc-Spain1H-MØ), or T. gondii PRU-RFP (T. gondii PRU-RFP-MØ) at the indicated MOIs. (A) Representative micrographs show MØ nuclear staining (Nuclei), Alexa-594-stained tachyzoites (Nc-Spain7), and fluorescent gelatin (Gelatin) with areas of gelatin degradation (absence of fluorescence signal). Arrowheads exemplify Nc-Spain7-infected MØs with an absence of gelatin degradation. Asterisks exemplify non-infected by-stander MØs and co-localization with gelatin degradation. Scale bar represents 100 μm. (B–D) Bar graphs show the mean (±SEM) relative gelatin degradation of unchallenged cells in CM (set to 1.0, MOI 0), non-infected by-stander cells (Bystander) and Neospora Spain7- (B), Spain1H- (C) or T. gondii-infected (D) cells (Infected). The analysis comprised 160 FOVs and a total of 15.579 ± 1.087 (SEM) cells per condition from two independent experiments. Significant differences are indicated (*p < 0.05).