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. 2019 Apr 30;10:915. doi: 10.3389/fimmu.2019.00915

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Copyright © 2019 García-Sánchez, Jiménez-Pelayo, Horcajo, Regidor-Cerrillo, Ólafsson, Bhandage, Barragan, Werling, Ortega-Mora and Collantes-Fernández.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ROS generation induced by N. caninum infection. (A) Representative histograms show the mean fluorescence intensity (MFI) determined by flow cytometry analysis of non-infected MØs (MØ), MØs challenged for 1 and 24 h with N. caninum Nc-Spain7 (Nc-Spain7-MØ), Nc-Spain1H (Nc-Spain1H-MØ), and heat-inactivated tachyzoites (Nc HI-MØ) stained with CellROX Green Reagent. (B) The bar graph show the comparison of the MFI between groups. Each column and error bar represents the mean and SD of three replicates from three independent experiments; Significant differences are indicated (*p < 0.05; **p < 0.01; ***p < 0.001).