Figure 9.

IFN-gamma production by lymphocytes in co-culture with N. caninum-infected MØs. The graph represents the concentration of IFN-γ in supernatants obtained from lymphocytes in co-culture with non-infected MØs (MØ) or MØs challenged with N. caninum Nc-Spain7 (Nc-Spain7-MØ), Nc-Spain1H (Nc-Spain1H-MØ), heat-inactivated tachyzoites (Nc HI-MØ) or 100 ng ml⁻¹ LPS (LPS-MØ). Each column and error bar represents the mean and SD of three replicates from three independent experiments. Significant differences are indicated (*p < 0.05; ***p < 0.001). IFN-γ was not detected in samples without lymphocytes, and lymphocytes without macrophages (lymphocytes) reached average IFN-γ values lower than 1,000 pg ml⁻¹.