Figure 3.

HDAC4 mutations export FoxO1 from nuclear to cytosol through increasing levels of acetylation in Min6 cells. a. Immunostaining of DAPI (blue), HDAC4(red), and FoxO1 (green) in MIN6 cells that were transfected with wild‐type (WT) or three mutated HDAC4 (p.H227R, p.D234N, and p.E374K). Both HDAC4 (left) and FoxO1 (right) were translocated into the cell cytosol in the mutated HDAC4 transfected cells compared to the wild type of HDAC4 transfected cells. b. Immunoblotting with antibodies against FoxO1, α‐Tubulin, and Histone 3 (H3) of lysates separately extracted from nuclear (left) and cytosol (right) of MIN6 cells that were transfected with wild‐type (WT) or three mutated HDAC4 (p.H227R, p.D234N and p.E374K). c. Immunoblotting with antibodies against acetylated‐lysine FoxO1, and α‐Tubulin using anti‐FoxO1 immunoprecipitation lysates of MIN6 cells that are transfected with wild‐type (WT) or three mutated HDAC4 (p.H227R, p.D234N, and p.E374K)