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. 2019 Apr 30;10:951. doi: 10.3389/fimmu.2019.00951

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Copyright © 2019 Staniek, Lorenzetti, Heller, Janowska, Schneider, Unger, Warnatz, Seidl, Venhoff, Thiel, Smulski and Rizzi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Both TRAIL-R1 and TRAIL-R2 mediate apoptosis in primary human B cells and tumor B cell lines. (A) Experimental approach, describing the addition of reagents to the culture to study TRAIL-induced apoptosis in BJAB cells by flow cytometry. (B) Percentages of live BJAB WT (left) and TRAIL-R2 KO (right) cells after 24h of scalar concentrations of TRAIL ± scalar concentrations of TRAIL-R1 blocking antibody (n = 3). (C) Experimental setup to analyze contribution of TRAIL-R1 and TRAIL-R2 to TRAIL-induced apoptosis in activated B cells by flow cytometry. (D) Representative identification of live primary B cells by FSC-A and SSC-A (left) and quantification (right) of live B cells after 24 h of TRAIL stimulation ± TRAIL-R1 blocking antibody (1μg/ml) at day 4 of culture by flow cytometry. Dots represent triplicates of four biological replicates. (B, D) Mean values ± SD are shown. (B) n, independent experiments. (D) ^*adj.p < 0.05; ^***adj.p < 0.001. Statistical analysis was performed using two-way ANOVA, followed by Tukey's multiple comparison test where appropriate.