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. 2019 Apr 30;10:942. doi: 10.3389/fimmu.2019.00942

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Copyright © 2019 Popugailo, Rotfogel, Supper, Hillman and Kaempfer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SEB triggers B7-1/CD28 synapse formation: contour plots. Contour plots are shown for a representative experiment in Figure 5A, upon incubation of cells expressing CD28/GFP with cells expressing B7-1/Cherry (A–D) or B7-2C/Cherry (E). Incubation was done in the presence of the indicated concentrations of SEB from 0 to 1 μg/ml; per cent doubly labeled cells is shown in upper righthand corner of each panel.