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. 2019 Apr 30;10:365. doi: 10.3389/fgene.2019.00365

FIGURE 1.

FIGURE 1

Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include MS2 phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.