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. 2019 Apr 30;10:541. doi: 10.3389/fpls.2019.00541

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Copyright © 2019 Yan, Wu, Li, Liu, Cui, Jiang, Ma, Luo and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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INV activities, sugar contents and starch accumulation in leaves of wild-type (WT) and the MeCWINV3-overexpressed (OE) cassava plants. (A–C) Contents of glucose (A), fructose (B), and sucrose (C) under a day/night cycle. (D) Relative transcription level of MeCWINV3. (E–G) The enzymatic activity assay of CWINV (E), VINV (F), and CINV (G). (H,I) Iodine staining of leaves collected at 6:00 a.m. (H) and their total starch contents (I). Different letters indicate significant differences (one-way ANOVA, p < 0.05).