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. 2019 Apr 30;10:512. doi: 10.3389/fphys.2019.00512

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Copyright © 2019 Pizzicannella, Gugliandolo, Orsini, Fontana, Ventrella, Mazzon, Bramanti, Diomede and Trubiani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vivo VEGFA and VEGFR2 expression. Immunofluorescence staining of VEGFA and VEGFR2 showed the presence of the protein in semithin section samples obtained after 6 weeks of grafting in rat calvaria. Panels (A1,A2, B1,B2, C1,C2, D1,D2, E1,E2) showed the expression of VEGFA. Panels (A3,A4, B3,B4, C3,C4, D3,D4, E3,E4) showed the expression of VEGFR2. (A1,A3) 3D-COL at green fluorescent channel, (A2,A4) 3D-COL at light transmission channel. (B1,B3) 3D-COL/hPDLSCs at green fluorescent channel, (B2,B4) 3D-COL /hPDLSCs at light transmission channel. (C1,C3) 3D-COL/hPDLSCs/CM at green fluorescent channel, (C2,C4) 3D-COL/hPDLSCs/CM at light transmission channel. (D1,D3) 3D-COL/hPDLSCs/EVs at green fluorescent channel, (D2,D4) 3D-COL/hPDLSCs/EVs at light transmission channel. (E1,E3) 3D-COL/hPDLSCs/PEI-EVs at green fluorescent channel, (E2,E4) 3D-COL/hPDLSCs/PEI-EVs at light transmission channel. Mag, 20X; C, mouse calvaria; ^∗3D-COL.