Table 6.
Cytotoxic action of individual fractions of AMPs and antibiotics toward normal or tumor mammalian cells and their hemolytic activity toward human erythrocytes.
| Sample | MECa (μM) of cytotoxic action toward | MECa (μM) of hemolysis of human erythrocytes | ||||
|---|---|---|---|---|---|---|
| Normal Cells | Tumor Cells | |||||
| Human PBMC | Human neutrophils | Murine peritoneal macrophages | K562 | Murine EAC | ||
| PG-1 | 5.0 | 2.5 | 2.5 | 1.25 | 1.25 | 2.0 |
| LL-37 | 12 | 10 | >20 [40] | 5.0 | 10 | 5.0 |
| Lysozyme | >50 [100] | >50 [100] | >50 [100] | >50 [100] | 50 | >50 [100] |
| ChBac3.4 | 20 | >20 [40] | >20 [40] | 20 | 5.0 | >50 [100] |
| Gentamicin | >50 [100] | 50 | >50 [100] | 50 | >50 [100] | >50 [100] |
| Rifampicin | >20 [40] | 20 | >20 [40] | >20 [40] | >20 [40] | 20 |
| Oxacillin | >50 [100] | >50 [100] | >50 [100] | >50 [100] | >50 [100] | 25 |
| Ofloxacin | >50 [100] | >50 [100] | >50 [100] | >50 [100] | >50 [100] | >50 [100] |
| Polymyxin B | 50 | >50 [100] | 50 | 25 | 50 | >50 [100] |
PBMC, peripheral blood mononuclear cells; EAC, Ehrlich ascites carcinoma.
Minimal effective concentrations (MEC) values are medians of 3–4 independent experiments made in triplicates. MECs are minimal concentrations where the statistically significant difference from the untreated (intact) cells is found with Mann-Whitney U-test (p < 0.01; n1 = 3, n2 = 6–8 for cytotoxic action; p < 0.05, n1, n2 = 3 for hemolysis). If actual MEC value was out of the tested concentrations range, it was assessed as twice the maximal tested concentration; the corresponding value is given in square brackets.