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. 2019 Apr 30;9:128. doi: 10.3389/fcimb.2019.00128

Table 6.

Cytotoxic action of individual fractions of AMPs and antibiotics toward normal or tumor mammalian cells and their hemolytic activity toward human erythrocytes.

Sample MECa (μM) of cytotoxic action toward MECa (μM) of hemolysis of human erythrocytes
Normal Cells Tumor Cells
Human PBMC Human neutrophils Murine peritoneal macrophages K562 Murine EAC
PG-1 5.0 2.5 2.5 1.25 1.25 2.0
LL-37 12 10 >20 [40] 5.0 10 5.0
Lysozyme >50 [100] >50 [100] >50 [100] >50 [100] 50 >50 [100]
ChBac3.4 20 >20 [40] >20 [40] 20 5.0 >50 [100]
Gentamicin >50 [100] 50 >50 [100] 50 >50 [100] >50 [100]
Rifampicin >20 [40] 20 >20 [40] >20 [40] >20 [40] 20
Oxacillin >50 [100] >50 [100] >50 [100] >50 [100] >50 [100] 25
Ofloxacin >50 [100] >50 [100] >50 [100] >50 [100] >50 [100] >50 [100]
Polymyxin B 50 >50 [100] 50 25 50 >50 [100]

PBMC, peripheral blood mononuclear cells; EAC, Ehrlich ascites carcinoma.

a

Minimal effective concentrations (MEC) values are medians of 3–4 independent experiments made in triplicates. MECs are minimal concentrations where the statistically significant difference from the untreated (intact) cells is found with Mann-Whitney U-test (p < 0.01; n1 = 3, n2 = 6–8 for cytotoxic action; p < 0.05, n1, n2 = 3 for hemolysis). If actual MEC value was out of the tested concentrations range, it was assessed as twice the maximal tested concentration; the corresponding value is given in square brackets.