Table 7.
Hemolytic action of combinations of AMP and conventional antibiotics toward human erythrocytes.
| A and B combination | Hemolytic activity | FECIa | |
|---|---|---|---|
| (½ MEC A + ½ MEC B) | (¼ MEC A + ¼ MEC B) | ||
| LL-37 and GEN | + – – [–] | – – – [–] | >1.0 |
| PG-1 and GEN | – – – [–] | – – – [–] | >1.0 |
| Lysozyme and GEN | – – – [–] | – – – [–] | >1.0 |
| Lysozyme and PMB | – – – [–] | – – – [–] | >1.0 |
| ChBac3.4 and OX | – – – [–] | – – – [–] | >1.0 |
| ChBac3.4 and OFL | – – – [–] | – – – [–] | >1.0 |
| ChBac3.4 and RIF | – – – [–] | – – – [–] | >1.0 |
GEN, gentamicin; PMB, polymyxin B; OX, oxacillin; OFL, ofloxacin; RIF, rifampicin. + | – presence or absence of the statistically significant difference from intact cells (Mann-Whitney U-test p < 0.05, n1, n2 = 3) in each of the three independent experiments; resulting assessment of the combination effect is given in square brackets.
Assessment of the minimal Fractional Effective Concentration Index (FECI) based on the results for (½ MEC A + ½ MEC B) and (¼ MEC A + ¼ MEC B) combinations of substances A and B; FECI > 1.0 shows independent action or antagonism, 0.5 < FECI ≤ 1 indicates additivity, FECI ≤ 0.5 denotes synergy. MEC, minimal effective concentration.