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Detection of toxigenic V. cholerae O1 by culture and PCR.
| Sample type | Sampling | Number of samples | Total ctxA-positive samplesa | Total ctxA-positive O1 samplesa | Total ctxA positive samples (APW-PCR)b |
|---|---|---|---|---|---|
| Phytoplankton | Horizontal method | 60 | 4 | 0 | 5 |
| Vertical method | 60 | 6 | 5 | 9 | |
| Water | Onshore | 60 | 4 | 0 | 4 |
| Offshore | 60 | 3 | 2 | 2 | |
| Carps | Landing sites | 120 | 5 | 3 | 3 |
aStrains isolated on TCBS agar and confirmed by PCR; bToxigenic V. cholerae identified by PCR of samples pre-enriched in alkaline peptone water (APW).
