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Table showing the starting electroporation condition (manufacture protocol) compared with the optimal condition determined for resting and IL-2 activated NK cells.
| No. of cells | First pulse | Second pulse | Wash buffer | Electroporation buffer | |
|---|---|---|---|---|---|
| Manufacture suggested | 2*107 /ml | 2100V / 20ms | _ | PBS 1X | Buffer R |
| Resting NK cells O.C. | 4*107 /ml | 2050V / 20ms | 500V/100ms | PBS 1X | Buffer R |
| Expanded NK cells O.C. | 4*107 /ml | 1820V / 20ms | 500V/100ms | Buffer CD | Buffer O |
