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. 2019 Apr 30;14:3015–3026. doi: 10.2147/IJN.S197824

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© 2019 Bai et al.

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Immunohistochemical evaluation of bone regeneration at 12 weeks post-implantation. Representative immunohistochemical staining images for detection of OCN expression in DBB granules around nascent bone tissues covered with PTFE membranes (A) or BTO/P(VDF-TrFE) nanocomposite membranes (B). (C) and (D) are enlargements of specific regions of (A) and (B) respectively. Black arrowheads denote the positive expression of OCN. Scale bars =100 μm for (A–B); Scale bars =50 μm for (C-D).

Abbreviations: OCN, osteocalcin; DBB, deproteinized bovine bone; NB, nascent bone; PTFE, polytetrafluoroethylene; BTO, BaTiO₃; P(VDF-TrFE), poly(vinylidene fluoridetrifluoroethylene).