Skip to main content
. 2019 Apr 30;10:933. doi: 10.3389/fmicb.2019.00933

Table 2.

List of target genes along with their PCR primer sequences and amplicon sizes.

Target gene PCR primer sequence (5′→3′) Amplicon (bp) References
PCR primers blaOXA-48 F: TTGGTGGCATCGATTATCGG 744 Salloum et al., 2015
R: GAGCACTTCTTTTGTGATGGC
blaNDM-1 F: GGAAACTGGCGACCAACG 678 Salloum et al., 2015
R: ATGCGGGCCGTATGAGTGA
blaOXA-23-like F: GATCGGATTGGAGAACCAGA 501 Woodford et al., 2006
R: ATTTCTGACCGCATTTCCAT
blaOXA-24/40 F: GGTTAGTTGGCCCCCTTAAA 246 Woodford et al., 2006
R: AGTTGAGCGAAAAGGGGATT
blaOXA-51-like F: TAATGCTTTGATCGGCCTTG 353 Woodford et al., 2006
R: TGGATTGCACTTCATCTTGG
blaOXA-58 F: AAGTATTGGGGCTTGTGCTG 599 Woodford et al., 2006
R: CCCCTCTGCGCTCTACATAC
blaOXA-143-like F: TGGCACTTTCAGCAGTTCCT 149 Higgins et al., 2010
R: TAATCTTGAGGGGGCCAACC
blaKPC-2 F: GCAGCGGCAGCAGTTTGTTGATT 184 Salloum et al., 2015
R: GTAGACGGCCAACACAATAGGTGC
RT-qPCR primers blaOXA-48 F: TTCGGCCACGGAGCAAATCAG 240 Salloum et al., 2015
R: GATGTGGGCATATCCATATTCATCGCA
blaNDM-1 F: TTGGCGATCTGGTTTTCC 195 Salloum et al., 2015
R: GGTTGATCTCCTG CTTGA
blaKPC-2 F: GCAGCGGCAGCAGTTTGTTGATT 184 Salloum et al., 2015
R: GTAGACGGCCAACACAATAGGTGC
rpoB F: TCGAAACGCCTGAAGGTC 184 Salloum et al., 2015
R: TTGGAGTTCGCCTGAGC