Table 2.
Target gene | PCR primer sequence (5′→3′) | Amplicon (bp) | References | |
---|---|---|---|---|
PCR primers | blaOXA-48 | F: TTGGTGGCATCGATTATCGG | 744 | Salloum et al., 2015 |
R: GAGCACTTCTTTTGTGATGGC | ||||
blaNDM-1 | F: GGAAACTGGCGACCAACG | 678 | Salloum et al., 2015 | |
R: ATGCGGGCCGTATGAGTGA | ||||
blaOXA-23-like | F: GATCGGATTGGAGAACCAGA | 501 | Woodford et al., 2006 | |
R: ATTTCTGACCGCATTTCCAT | ||||
blaOXA-24/40 | F: GGTTAGTTGGCCCCCTTAAA | 246 | Woodford et al., 2006 | |
R: AGTTGAGCGAAAAGGGGATT | ||||
blaOXA-51-like | F: TAATGCTTTGATCGGCCTTG | 353 | Woodford et al., 2006 | |
R: TGGATTGCACTTCATCTTGG | ||||
blaOXA-58 | F: AAGTATTGGGGCTTGTGCTG | 599 | Woodford et al., 2006 | |
R: CCCCTCTGCGCTCTACATAC | ||||
blaOXA-143-like | F: TGGCACTTTCAGCAGTTCCT | 149 | Higgins et al., 2010 | |
R: TAATCTTGAGGGGGCCAACC | ||||
blaKPC-2 | F: GCAGCGGCAGCAGTTTGTTGATT | 184 | Salloum et al., 2015 | |
R: GTAGACGGCCAACACAATAGGTGC | ||||
RT-qPCR primers | blaOXA-48 | F: TTCGGCCACGGAGCAAATCAG | 240 | Salloum et al., 2015 |
R: GATGTGGGCATATCCATATTCATCGCA | ||||
blaNDM-1 | F: TTGGCGATCTGGTTTTCC | 195 | Salloum et al., 2015 | |
R: GGTTGATCTCCTG CTTGA | ||||
blaKPC-2 | F: GCAGCGGCAGCAGTTTGTTGATT | 184 | Salloum et al., 2015 | |
R: GTAGACGGCCAACACAATAGGTGC | ||||
rpoB | F: TCGAAACGCCTGAAGGTC | 184 | Salloum et al., 2015 | |
R: TTGGAGTTCGCCTGAGC | ||||