Fig. 2.

TMEFF2 silencing in PCa cells induces androgen-driven expression of cell cycle genes. a Western Blot analysis to determine knockdown of TMEFF2 in LNCaP cells using three different TMEFF2 targeted shRNAs. Only sh_TMEFF2–1 and sh_TMEFF2–2 appreciably silenced TMEFF2 expression. Note that Tmeff2 is an androgen-regulated gene. Representative blot from > 3 repeats. b qRT-PCR data in the LNCaP-sh_TMEFF2 cells confirming increased expression in response to androgen stimulation of the cell cycle genes selected for the TMCC11 signature. Data is the average of 3 independent repeats and was analyzed using T-test. Error bars correspond to s.e.m. c Clustering analysis of TMCC11 signature genes in the MSKCC cohort. Each column corresponds to an individual patient. The status of some clinicopathological variables for each sample has been included in the figure at the top of the heatmap. *P < 0.05; **P < 0.01