Fig. 6.

Hibiscus extract enhances chemotherapeutic ability to reduce mitochondrial membrane potential. a MDA-MB-231 (left) MCF-7 (right) breast cancer cells were treated with chemotherapeutics taxol and cisplatin individually and in combination with 1 mg/mL HE and assessed at 48 h. Results were obtained using image-based cytometry to assess the percentage of cells positive with fluorescence associated with mitochondrial membrane potential (TMRM, fluoresces red). Values are expressed as a mean ± SD from three independent experiments. b Fluorescence microscopy images of individual and hibiscus combination chemotherapeutic treatments on MDA-MB-231 and MCF-7 cells were taken at 48 h.. Fluorescent images stained with TMRM (red) and Hoechst (blue) at 400× magnification. Images are representative of three independent experiments. Statistical calculations were performed using Two-Way ANOVA multiple comparison. *p < 0.05 vs. Control, **p < 0.01 vs. Control, ****p < 0.0001 vs. Control, @p < 0.05 vs. Individual Chemotherapy Treatment