Fig. 7.

Cells interaction analysis. a A cell preparation treated with CD90 antibodies before hybridization. b–d FICTION analysis. Cell preparations treated with CD90 antibodies and hybridized with the 500 TAMRA-labeled DNA fragment corresponding to the rearranged VDJ locus of the clonotypic B cell. DAPI staining of nuclei. Arrows indicate specific hybridization sites, mesenchymal stem cells (CD90 marker), and areas of ectopic contacts of clonotypic B cells and mesenchymal stem cells. e Transmission electron microscopy analysis. 1—Typical contact interactions between cells in sphere structure are shown by arrows. In these cases, one of the cells contacts the neighboring cells through cytoplasmic processes. 2—Group of cells with a typical morphology: increased nuclear-cytoplasmic ratio, presence of two nuclei in individual cells, high euchromatin content in nuclei, polar non-uniform distribution of organelles in the cytoplasm. The site of cell membranes fusion in the form of cytoplasmic bridges is shown by arrow