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. 2019 May 7;20:343. doi: 10.1186/s12864-019-5693-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Real-time PCR identification of selected candidate genes related to anthocyanin biosynthesis. There were seven candidate genes including five transcription factors which might regulate anthocyanin, BIM2, MYB21, bHLH30, ARALYDRAFT (ARRI) and bHLH49; and E3 ubiquitin-protein ligase and Alpha-xylosidase. Significant differences of Xianghongtai01 compared withYinong50D are indicated by * based on the Students t test, (*P < 0.05, ** P < 0.01; ***P < 0.001). The transcript levels of the genes are normalized to actin transcript level and expressed relative to PPIS which is set to 1. In all cases means ± SD (n = 3) are represented