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Validation of RNA-seq results by quantitative reverse transcription polymerase chain reaction (qPCR). Porcine GAPDH gene is used as the endogenous control. Expression was quantified using the comparative cycle threshold (2−ΔΔCt) value method. The data were expressed as the mean ± SEM). An asterisk denotes a significant difference (*P < 0.05; **P < 0.01).
