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. Author manuscript; available in PMC: 2019 May 7.
Published in final edited form as: Cell Rep. 2019 Mar 12;26(11):3037–3050.e4. doi: 10.1016/j.celrep.2019.02.052

Figure 5. SHOC2 Binds to Raptor and Competitively Inhibits Raptor-mTOR Binding.

Figure 5.

(A–C) H1299 (A) or Huh7 (B and C) cell lysates were prepared for IP, followed by IB with indicated Abs.

(D–G) H1299 (D and F) or A549 (E and G) cells were transfected with indicated plasmids or siRNA oligos for 48 h, followed by IP or IB with indicated Abs.

(H and I) 293 cells were transfected with indicated plasmids of SHOC2 (H) or Raptor (I) for 48 h, followed by IP and IB with indicated Abs. The domain structure of Raptor is shown (I, top).

(J and K) H1299 cells were transfected with indicated plasmids for 48 h, followed by IB (J), or staining with Cyto-ID Autophagy detection kit (K). Shown are mean ± SEM from three independent experiments, **p < 0.01, ***p < 0.001.