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. 2019 Apr 30;10:933. doi: 10.3389/fimmu.2019.00933

Figure 2.

Figure 2

BET inhibitors reduce cytokine-induced inflammatory mediator production by telomerase-immortalized gingival keratinocytes (TIGKs) without affecting cell viability. (A) Relative mRNA expression of IL8, IL6, IL1B, CXCL10, MMP9, and CCL20 in TIGKs treated with DMSO or 1 μM I-BET151 for 30 min prior to simulation with TNF or IL-1β (both at 10 ng/ml) for 4 h determined by qPCR (mean + SEM; n = 4–5). *P < 0.05, **P < 0.01, ***P < 0.001; ratio paired t-test. (B) IL-6, IL-8, and CCL20 production by TIGKs treated with DMSO or increasing concentrations (100 nM, 500 nM, and 1 μM) of I-BET151 or JQ1 for 30 min followed by simulation with 10 ng/ml TNF (left panel) or 10 ng/ml IL-1β (right panel) for 24 h measured by ELISA (mean concentration + SEM; n = 5). (C) Viability of TIGKs (n = 3) treated with DMSO or 1 μM I-BET151 for 30 min prior to simulation with TNF or IL-1β (both 10 ng/ml) for 24 h analyzed using MTT assay and shown as % of control + SEM.