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. 2019 Apr 30;10:933. doi: 10.3389/fimmu.2019.00933

Figure 4.

Figure 4

BET inhibitors suppress production of inflammatory mediators by gingival fibroblasts (GFs) infected with P. gingivalis. (A) qPCR analysis of relative mRNA expression of IL8, IL6, IL1B, CCL2, CCL5, MMP3, COX2, and CCL20 in GFs treated with DMSO, I-BET151 or JQ1 (both at 1 μM) for 30 min prior to infection with P. gingivalis (MOI = 100) for 4 h (mean + SEM; n = 5–6). (B) Production of IL-8, IL-6 and CCL2 by GFs exposed to DMSO or I-BET151 or JQ1 at two different concentrations (100 nM and 1 μM) for 30 min before P. gingivalis infection (MOI = 100) for 1 h, followed by washing and 24 h culture in fresh medium containing DMSO or BET inhibitors (mean concentration + SEM; n = 5–6). (A,B) *P < 0.05, **P < 0.01, ***P < 0.001; one-way ANOVA followed by Bonferroni multiple comparison test. (C) Intracellular survival of P. gingivalis in GFs treated with DMSO or 1 μM I-BET151 for 20 h prior to infection with P. gingivalis (MOI = 100) for 1 h determined by colony-forming assay immediately (1 h) or 24 h post-infection. Data are presented as mean colony-forming units (CFU)/cell + SEM of 4 independent experiments.