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. 2019 Apr 30;10:520. doi: 10.3389/fphys.2019.00520

FIGURE 5.

FIGURE 5

Reduced phosphorylation levels of Cardiac Ryanodine Receptor at Ser-2814 underlie its dysfunctional activity. (A) Representative Western Blot images of phosphorylated RyR2 at Ser2808 (pS2808-RyR2), Ser2814 (pS2814-RyR2), and total ryanodine receptor (RyR2) in whole heart homogenates of control (C) and MetS rats. GAPDH signals were used as loading controls. 50 μg of protein from whole heart homogenates of each experimental group (C and MetS) were subjected to 4–16% gradient SDS-PAGE, blotted onto PVDF membranes during 2 h, and probed with the respective primary and secondary antibodies. Bar graphs indicate normalized expression levels of total RyR2 (B), pS2808RyR2/RyR2 total (C), and pS2814RyR2/RyR2 total (D) in whole heart homogenates of control (C, white bars) and MetS (orange bars) rats. N = 6–7 heart preparations for each experimental group. All values in graphs are presented as Mean ± SEM. *P < 0.05 vs. control values.