TABLE 3.

Ca²⁺-dependence of [³H]-ryanodine binding in microsomes of MetS and controls hearts.

Condition	Bmax (pmol [3H]ryanodine/mg Pt)	Ka (μM)	Ki (mM)	C (pmol [3H]ryanodine/mg Pt)	N
Control	0.19 ± 0.01	8.48 ± 1.05	7.6 ± 0.6	0.013 ± 0.001	9
MetS	0.14 ± 0.08*	7.57 ± 0.86	5.2 ± 1.0	0.009 ± 0.003	6

Values are Mean ± SEM of indicated independent determinations for each experimental group. Bmax indicates the maximum number of active RyRs, K${}_a$ and K${}_i$ are Hill activation and inactivation constants, respectively; and C is an initial [${}^3$H]-ryanodine binding value at very low [Ca${}^{2+}$] (pCa8). N indicates the number of hearts. *P < 0.05 vs. control group.