A. Etamicastat (Etam, 1O mg/kg) or vehicle was administered by gavage. Blood pressure was measured under pentobarbital anesthesia 9 and 18 h after etamicastat administration in different groups of D2+/+ and D2−/− mice. n=4–5/group; *P<O.O5 vs all others, one-way ANOVA followed by Holm-Sidak test
B. Etamicastat (Etam, 1O mg/kg/day) or vehicle was added to the drinking water. Blood pressure was measured by telemetry in conscious mice. Values are means of 6 h measurements (one per h). D2+/+ n=5; D2−/− n=4. *P<O.O5 vs D2+/+ one-way ANOVA followed by Holm-Sidak test.
C. Etamicastat (1O or 5O mg/kg/day) was added to the drinking water. Blood pressure was measured by telemetry in conscious mice. Values shown are means of 6 hr measurements during the day and night (one reading per h after 5 days on each treatment). D2+/+ n=4; D2−/− n=3. * P<O.O5 vs D2−/− vehicle or same treatment D2+/+, two-way ANOVA followed by Holm-Sidak test.