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. 2019 Mar 29;11(7):1965–1976. doi: 10.18632/aging.101885

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Copyright © 2019 Ge et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SIRT6 expression in aged mouse oocytes and embryos. Fully-grown oocytes and two-cell embryos from young and aged mouse were collected to evaluate SIRT6 expression. (A) Quantitative RT-PCR showing the lowered SIRT6 mRNA levels in aged oocytes and two-cell embryos. (B–C) SIRT6 protein expression in aged oocytes and two-cell embryos was verified by immunoblotting. Tubulin served as a loading control. Band intensity was calculated using ImageJ software, and the ratio of SIRT6/tubulin expression was normalized and values are indicated. Data are expressed as mean percentage ± SD of three independent experiments. *P <0.05, **P <0.01.