Figure 5.

SIRT6 overexpression in aged oocytes increases the telomere length in early embryos. (A) Schematic presentation of the SIRT6 overexpression experiments. (B) Efficiency of SIRT6 overexpression (SIRT6-OE) after mRNA injection was verified by immunoblotting. (C) Relative telomere length expressed in two-cell embryos is expressed as a T/S ratio determined by quantitative RT-PCR analysis. Data are expressed as mean percentage ± SD of three independent experiments (n=86 for young; n=85 for old; n=80 for old+SIRT6-OE). **P <0.01.