Figure 3.

Treatment of Plasmodium cultures with UA derivatives with no effect on microgametogenesis. (a) Blood from mice infected with P. berghei ANKA was mixed with DMSO, aphidicolin, BT37, or BT122 in ookinete media, incubated for 15 min, and then analyzed by microscopy for exflagellation. For each treatment group, the number of exflagellation centers was scored for five fields per replicate and three replicates per mouse (M1–M3). The arithmetic means for all replicates are reported. (b) For P. falciparum (NF54), the same assay was performed using gametocyte cultures (C1–C3) instead of mice. As in a, arithmetic means are reported for all replicates.