Fig. 5.

Cry1Ca plasma membrane binding is not affected by the presence of chelators. Sf9wt cells (left row) or Sf9-LD₈₀ cells (right row) were incubated for 40 min in complete media containing 3 mg/l of Cry1Ca toxin alone (+Tox), Cry1Ca toxin +5 mM EDTA (+Tox +EDTA) or Cry1Ca toxin +5 mM EGTA (+Tox +EGTA). Immunostaining was performed using primary antibodies to Cry1Ca and secondary antibodies coupled to Alexa Fluor 488. Toxin localization was visualized using an inverted epifluorescence microscope. As a control (CT), immunostaining was carried out onto cells incubated without toxin. The numbers indicate the percentage of cells immunostained. Shown results are those from one experiment representative of two independent experiments. Scale bar: 20 µm.