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. 2019 Apr 15;8(4):bio042168. doi: 10.1242/bio.042168

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — NSC-TAF or TRF2-depleted NSCs exhibit defective cell polarity but do not express differentiation markers. (A) Taf4^LL07382 NSCs or (B) Taf7^LL07382 NSCs fail to express normal levels of Insc. Mitotic cells are labeled with phospho Histone H3 (pH3), CD8:GFP (a membrane-tethered GFP) labels the clones and Insc is shown in red. (C) Knockdown of TBP, TRF2 or TAF9 does not result in nuclear accumulation of Prospero. (D) Knockdown of TBP, TRF2 or TAF9 does not result in nuclear accumulation of Elav. The white dotted line demarcates the optic lobe (left)/central brain (right) boundary. In both C and D, transgenes were expressed using a UAS-Dcr2; inscGAL4, UAS-CD8:GFP, tubGAL80^ts driver and transgenes induced for 72 h. Scale bars: 10 µm (A–C); 20 µm (D).