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. 2019 Apr 5;8(4):bio039917. doi: 10.1242/bio.039917

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Identification of stage-specific genes and first-expressed genes. (A) Histogram of the number of stage-specific genes in NT-LW, NT-D and IV-D embryos. Stage-specific genes are defined as those expressed solely during one stage. (B) Venn diagram for the stage-specific genes in the three groups of blastocysts. (C) Histogram of the number of genes activated at the respective embryonic stages as detected by first appearance of specific transcripts. (D,E) Venn diagrams for the first-expressed genes in the three groups of embryos at the 4-cell and 8-cell stages, respectively.