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. 2019 May 7;6(2):025006. doi: 10.1117/1.NPh.6.2.025006

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© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Fig. 1 — Comparison of Luc2, CBG99, PpyRE9, x5g, x5r, and hRluc emission spectra in vitro. Plotted is the photon emission from $EF 1 α$ -luciferase-T2A-copGFP transfected HEK 293T cells recorded with the IVIS Spectrum CT system (mean of $n = 4$ to 6). Photon emission has been normalized to the individual copGFP expression. Graph with gray background is a zoom-in for 580 to 700 nm to highlight the differences in the red part of the spectra.