Table 1. Oligonucleotides for NS5A gene amplification of HCV GTs.
| Genotype | Technique | NS5A Primers | Sequence (5’–3’) | Genome position | Reference |
|---|---|---|---|---|---|
| 1a | PCR 1 | NS5A_F1 | CAGTGCARTGGATGAACCG | 6076–6094 | [14] |
| NS5A_R1 | CGAGTTGCTCAGTGCGTT | 7671–7688 | -- | ||
| PCR 2 | NS5A_F1 | CAGTGCARTGGATGAACCG | 6076–6094 | [14] | |
| NS5A_R2 | TARGACATYGAGCARCACAC | 7593–7612 | [14] | ||
| 1b | PCR 1 | NS5A_F1 | CAGTGCARTGGATGAACCG | 6076–6094 | [14] |
| NS5A_R3 | GTCTGTCAAATGTGACTTTCTTCT | 7747–7770 | [14] | ||
| PCR 2 | NS5A_F2 | CGGCTGATAGCGTTCG | 6093–6108 | [14] | |
| NS5A_R2 | TARGACATYGAGCARCACAC | 7593–7612 | [14] | ||
| 3a | PCR 1 | NS5A_F4 | CAGTGGATGAACAGGCTCAT | 6097–6116 | -- |
| NS5A_R4 | CCTCAGCACTACATGGTGT | 7663–7681 | -- | ||
| PCR 2 | NS5A_F5 | GTGGATCAATGAAGACTACCC | 6243–6263 | -- | |
| NS5A_R5 | CCTCAGCACTACATGGTGT | 7663–7681 | -- |