Table 2. Oligonucleotides for NS5B gene amplification of HCV GTs.

Genotype	Technique	NS5B Primers	Sequence (5’–3’)	Genome position	Reference
1a	PCR 1	NS5B_F1	CTYAGCGACGGRTCRT	7539–7554	[15]
		NS5B_R1	TCACGGGTRAGGTARTAGAC	8742–8761	[15]
	PCR 2	NS5B_F2	TCGTGTGYTGCTCRATG	7591–7607	[15]
		NS5B_R2	TACCTGGTCATAGCCTCC	8621–8638	[15]
1b	PCR 1	NS5B_F3	TCYTGGTCTACYGTRAG	7551–7567	[15]
		NS5B_R3	AGGARCATGATGTTATCARCTC	8679–8700	[15]
	PCR 2	NS5B_F3	TCYTGGTCTACYGTRAG	7551–7567	[15]
		NS5B_R4	CCTAGTCATAGCCTCCGT	8616–8633	[15]
3a	PCR 1	NS5B_F5	TCTATGTCGTACTCTTGGACCG	7630–7651	--
		NS5B_R5	GGAGTAGGCAAAGCAGCAAAT	9341–9361	--
	PCR 2	NS5B_F5	TCTATGTCGTACTCTTGGACCG	7630–7651	--
		NS5B_R6	CGATCAAGTATCTCCTGGGATTG	8929–8951	--